In Vitro fertilization

En Amnios ofrecemos a nuestros pacientes las más elevadas probabilidades de gestación

In Vitro fertilization

En Amnios ofrecemos a nuestros pacientes las más elevadas probabilidades de gestación


What is in vitro fertilization?

In vitro fertilization (IVF) is a highly complex assisted reproduction treatment. This procedure consists of fertilizing the eggs outside the uterus (in vitro) with a semen sample prepared in the laboratory. Once the embryos are obtained, the highest quality embryos are transferred to the uterine cavity with the aim of achieving pregnancy. Fertilization or in vitro fertilization is a technique that provides high success rates. The process requires highly qualified professionals to carry it out, the use of the latest technologies in pregnancy and a faithful commitment on the part of the patient to follow all the steps.
Qué es la Fecundación in vitro

In which cases is in vitro fertilization indicated?

This treatment is recommended when there are causes that prevent adequate fertilization by natural means. In addition, at Amnios we carry out a preliminary study on all our patients, not only to determine the probable cause , but also to define and carry out the best process based on each case. Before beginning the procedure, patients are explained in detail what it means, what it consists of, and how each stage is performed.
The most common cases in which it is recommended are the following:

A disease in which the tissue that lines the inside of the uterus grows outside of the uterus. Its symptoms include pelvic pain and infertility, its cause is unknown and it can cause alterations in the fallopian tubes and low ovarian quality.

Advanced age

From the age of 35, the quality and number of ovules begin to decrease significantly in women. There is no defined age limit because there are many factors involved, but it is usually the main cause of poor-quality eggs.

Failures in previous cycles of artificial insemination

The reasons for these previous failures can be due to various reasons, from being related to the lack of ovules to the difficulty in having carried the pregnancy to a successful conclusion.

Malfunction in the fallopian tubes

In this case they may be blocked or have suffered damage or loss due to an ectopic pregnancy. Obstruction of the tubes is one of the main reasons for infertility in women since its proper functioning is essential for the union of the egg with the sperm. Often the obstruction consists of infections, endometriosis, or hydrosalpinx.


It is an alteration of the reproductive system usually caused by an infection and is characterized by the obstruction and accumulation of fluid in the tubes, which causes them to dilate and distend, causing infertility. In the case of having a hydrosalpinx, it is indicated to perform a small surgery to remove the damaged tube or tubes before the start of any IVF treatment since the results in terms of pregnancy rates can decrease significantly.

Preimplantation genetic diagnosis

These are the cases in which the embryo must undergo a preimplantation diagnosis to rule out genetic diseases, in this way the genetically altered embryo is detected before being transferred. This prevents the transmission of serious diseases such as cystic fibrosis or muscular dystrophy, among others, but it is also possible to carry out a genetic study of aneuploidies in the embryo, in order to rule out embryos affected by Down syndrome or other genetic pathologies linked to maternal age. This aneuploidy study is indicated in cases of recurrent miscarriage or embryo implantation failure.

Low quality of the sperm sample

Those cases in which the semen has a low number of useful sperm. This greatly decreases the chances of fertilization of the egg and therefore of achieving pregnancy and a viable pregnancy and could end in miscarriage. Sometimes the decrease in the quality of the semen sample can be associated with symptoms such as difficulty maintaining an erection, pain or testicular swelling. The causes of the drop in the quality of the semen sample are often unknown, but there may be environmental or genetic factors involved. Infections, varicocele, tumors or previous surgeries, as well as environmental factors such as tobacco, caffeine, very exhausting sports, certain medications or professions in which there is contact with toxins can have a very important influence on the reproductive level.

First consultation

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Is there an age limit to get pregnant?

In general terms and on the recommendation of the National Assisted Reproduction Commission, assisted reproduction techniques are not recommended for women over 50 years of age. However, the age limit depends on various factors, which are evaluated by Amnios In Vitro Project specialists.

Stages and duration of in vitro fertilization

All couples ask how long IVF lasts before undergoing treatment. To carry out all the stages of this assisted reproduction technique, approximately 30 days are necessary. The treatment phases are:
Ovarian stimulation:

In this first phase, hormonal drugs (called gonadotropins) are administered to stimulate the growth of ovarian follicles. Gonadotropic hormones used are very similar to natural ones: in fact, they are the analogue substances (FSH –follicle-stimulating hormone-, LH-luteinizing hormone) produced by the pituitary gland in our body to stimulate the growth of ovarian follicles. Ovarian follicles are small sacs filled with fluid that are placed inside a woman’s ovaries and they contain the immature egg. The aim of ovarian stimulation is to promote the growth and the ovulation of multiple follicles to retrieve multiple eggs. Gonadotropins are administered by subcutaneous injections, in a simple and painless way, and they can be administered by the patient herself after a proper explanation by our Nursing team. During the natural ovulatory cycle, ovaries usually produce a single follicle, containing just one egg. On this basis, ovarian stimulation is performed to increase the number of mature eggs to retrieve and consequently the chances to obtain a pregnancy. Ovarian stimulation must start at the beginning of the menstrual cycle. During the second or third day of the cycle, a transvaginal ultrasound is performed to check the follicles. In addition, a blood test is often performed to determine certain hormone levels such as LH, to evaluate the ovary status. If the ovaries result at rest, the administration of the medication can begin. Four or five days after, a new ultrasound is performed to check the response of the follicles to the hormonal stimulation.

Egg retrieval:

Once the follicles reach the proper growth, the following phase is the egg retrieval. During this procedure, an ultrasound probe is inserted into the vagina to identify follicles and a needle is guided through the vagina and into the follicles. The eggs are removed from the follicle through the needle, which is connected to a suction device. Once the content of the follicle (follicular fluid) is obtained, it is immediately delivered to the embryologists in the laboratory, who check under a microscope the presence of the eggs into the follicular fluid. The whole procedure lasts approximately 10 minutes, and usually the number of eggs obtained in each cycle range from 5 to 15. Patients are under mild sedation throughout all the procedure to not feel any discomfort of pain. Overall, it is a simple and safe procedure but, for some patients, could be stressful if they has never been in a surgery room before. Before the egg retrieval, the patient must be in a complete fasting state: she should not eat or drink for at least six hours before the procedure. After the procedure, it is advisable to rest for 1-2 hours to avoid possible side effects, which are usually rare. Typically, the patient does not suffer any discomfort after the intervention.

Egg fertilization

Once the eggs are obtained, there are two techniques to fertilize them: In Vitro Fertilization or Intracytoplasmic Sperm Injection (IVF or ICSI). In conventional IVF, the oocytes are placed in the same culture dish with a large number of spermatozoa (about 100,000 per oocyte) and incubated with the intention that the gametes themselves carry out the fertilization spontaneously as would happen in the female genital tract. To obtain a successful fertilization in conventional IVF it is required an adequate sperm quality: recovery of more than 5 million spermatozoa, with a minimum of 30% progressive motility. If these conditions are not satisfied, the chances of failure (fertilization failure) are higher. Thus, in cases of severe male infertility, ICSI would be the best choice. Intracytoplasmic Sperm Injection (ICSI) is a technique which involves injecting a one single good quality selected spermatozoa directly into the patient’s oocyte. The procedure is performed using very fine micro-manipulation equipment. ICSI is generally used in cases of low sperm quality, fertilization failures with conventional IVF, patients affected by infectious diseases transmissible by sperm, frozen oocytes or to perform pre-implantation genetic diagnosis of the embryo. Regardless of the technique used to fertilize the oocytes, the following day after the insemination embryologists check on the status of the fertilized eggs. Usually, 60-70% of the eggs are fertilized (either with IVF or with ICSI). The embryos are always kept in the incubators, to maintain strict culture conditions (controlling temperature, CO2 concentration, oxygen pressure, etc.). On the second day, the fertilized embryos with a normal evolution have divided and have between 2 and 4 cells; on the third day, they have between 6 and 8 cells and so on until the 5th-6th day when they should have already reached the blastocyst stage. The blastocyst stage is important because at this time the embryo start attaching to the uterus wall in the natural pregnancy, therefore it represents the last stage during which the embryo can be cultured outside the maternal environment. In Amnios we usually keep the embryos in culture in the laboratory until the fifth day. This is a procedure called blastocyst culture or long culture. In this way we obtain more information about their implantation potential, to increase pregnancy rates.

Embryo transfer

Embryo transfer is the last step of an in vitro fertilization cycle and is usually performed on the fifth or sixth day after ovarian pick-up. During this procedure, the embryo is loaded into a small catheter tube and eventually released in the uterus under ultrasound control. Embryo transfer is not painful: it is very similar to any gynecological examination and does not require sedation or analgesia. Once the catheter is withdrawn, the biologist checks the presence of the embryo, to assure that the embryos has been correctly released in the uterus). During the following 14 days, the woman undergoes a hormonal treatment (and sometimes with estrogen as well) that is introduced into the vagina to help the embryos implant. And 14 days after the transfer (earlier if we have transferred the embryos in the blastocyst phase), we can perform the blood pregnancy test. The decision of how many embryos to transfer is made by the specialists depending on each couple and current legislation, but it is advisable to transfer the embryos one by one. In the cases of non-transferred embryos, they can be vitrified to be used in subsequent cycles. Rest is not needed after embryo transfer. In vitro fertilization has no consequences on the health of the woman, and normal life can be led after it. It is possible to lead a completely normal life, although without great effort. It is not necessary to stay in bed or special care.

The right moment to do the pregnancy test is a common question asked by women who undergo in vitro insemination. The hormone called human chorionic gonadotropin (hCG) is the one who has the answer to this question. A laboratory analysis, with a blood sample taken between 12 and 14 days after the transfer, will reveal if the woman is pregnant.

When do you know you are pregnant?

Success rates

At Amnios we offer our patients the highest odds. The success rate is above 55-60% for each transfer of a blastocyst in cases with a good prognosis. It is a very effective technique, with an increasing percentage of pregnancies achieved on the first attempt. Children born by in vitro fertilization in our country are increasing in number and every year the number of couples who opt for this procedure grows. The testimonials of these couples are very good and invite others to opt for this assisted reproduction technique. A good previous investigation, the age of the woman, the exhaustive assessment of sperm quality, the quality of the oocytes and the experience of the professionals who carry out the process are factors that have a decisive influence on the success of infertility. Vitro
The cost of this technique in our country can vary due to multiple factors. When choosing a clinic to carry out the treatment, it is convenient to consider not only the previous study that defines which technique is the most appropriate, but also the safety, experience and technologies used. For example, the WITNESS method used in Amnios for the continuous monitoring and traceability of gametes and embryos, which offers a guarantee of maximum traceability of our processes. Likewise, we offer affordable prices, payment and financing facilities, and collaboration with various medical societies.

What is the price of an in vitro fertilization?

Not your first cycle? don't be discouraged

If you have already undergone an In Vitro Fertilization cycle and have not achieved the expected success, do not be discouraged. That first cycle can give us a lot of information about how your ovaries and your eggs are working, and about what happens when eggs and sperm meet. All this experience can be of great help to improve the procedures of subsequent cycles and improve the chances of pregnancy. Come to a free consultation at AMNIOS, where we will assess how to improve your treatment and its probabilities

Advantages and disadvantages of in vitro fertilization

There are many arguments in favor without the need to fall into its ethical implications. It is a technique with many advantages:
  • It offers high pregnancy rates and is the ideal solution for many couples with fertility problems
  • It is a safe and effective technique
  • Through the technology used and the personalized treatment, the reproductive prognosis of the couple can be improved
  • It works in elderly women and men with a poor reproductive prognosis
  • Another of its advantages is that it allows you to avoid genetic diseases
Regarding the disadvantages of in vitro fertilization, it should be noted that this technique has practically no drawbacks, if the couple has been fully and adequately informed about the procedure and its reproductive prognosis at each age and is carried out by specialists with Demonstrated experience in this type of treatment. Anyone looking for the best clinic to undergo in vitro fertilization treatment in Spain should know us, as we are a reference for assisted reproduction clinics throughout the country and in Madrid.

Can you have twins through in vitro fertilization?

If it is the desire of the couple, this procedure can achieve a pregnancy of twins or twins and is more likely than in a natural pregnancy. In any case, it is the patient and her partner, in agreement with the specialist who treats them as a couple, who have the last word on the number of embryos to be transferred, always taking into account that multiple gestations imply a greater number of complications such as premature birth, and the possibility of hypertension and diabetes in pregnancy as well as intrauterine growth retardation.
To answer this question you have to understand the differences between the two , the most important one refers to where fertilization occurs . In artificial insemination it is in the tubes, it is the most natural procedure that is performed and is performed in cases of good prognosis and with a maternal age below 37-38 years. In vitro fertilization is carried out in a laboratory. and, later, it is inserted into the mother’s body once the embryo is constituted. It is a more complex technique and indicated for entry into women or couples over 37-38 years of age or who present genetic problems, previous artificial insemination failures, bilateral tubal obstruction, low quality of the semen sample, embryo implantation failure or miscarriages. repeat.

What is better, artificial insemination or in vitro fertilization?

Have you already decided?

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